ABSTRACT
A toxoplasmose é uma infecção cosmopolita com cerca de 10-20% dos indivíduos infectados desenvolvendo a forma clínica. O estudo de populações de Toxoplasma gondii é de importância médica e epidemiológica. Estudos anteriores correlacionam a progressão e a severidade das formas sintomáticas da toxoplasmose com os genótipos de T. gondii. Este estudo analisou amostras de necropsias de uma coorte de 15 pacientes que foram a óbito por desenvolverem a toxoplasmose disseminada. Foi um estudo restropectivo, na qual os dados clínico-laboratoriais eram conhecidos. A hipótese era que estes pacientes poderiam albergar parasitas altamente virulentos pela condição em que eles foram a óbito. As moléculas de DNA isoladas das necropsias foram utilizadas para determinar as características genotípicas de T. gondii...
Toxoplasmosis is a cosmopolitan infection. Around 10-20% of infected individuals develop the clinical form. The study of Toxoplasma gondii populations is medical and epidemiological importance. Previous studies correlated the progression and severity of the symptomatic forms in toxoplasmosis with T. gondii genotypes.This study analyzed necropsy samples from a cohort of 15 patients who died due to develop disseminated toxoplasmosis. In this restropspective study the clinical and laboratory data from patients were known. The hypothesis was that these patients were infected with highly virulent parasites by the condition in which they died. DNA molecules isolated from patient necropsies were used to determine T. gondii.genotypes...
Subject(s)
Toxoplasma , ToxoplasmosisABSTRACT
This study investigated the genetic features of T. gondii isolated directly in autopsies of HIV-infected patients who died with severe disseminated toxoplasmosis. This retrospective analysis was conducted in a cohort of 15 HIV-infected patients with clinical and laboratory data. They had previous cerebral toxoplasmosis at least 6 months before the disseminated toxoplasmosis episode. The hypothesis was that they were infected with highly virulent parasites due to the condition in which they died. T. gondii genotyping was done directly in DNA extracted from 30 autopsy brain and lung samples (2 per patient) and mutilocus PCR-RFLP genotyping was done using 12 molecular markers. The 30 clinical samples were genotyped successfully in 8 or more loci and six suggestive genotypes were identified. One of them was Toxo DB #11, previously identified in different domestic animals and virulent in experimental animals. The other five suggestive genotypes...(AU)